Research

Research Overview

The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement of analytes of interest into ultrasmall containers have greatly enhanced the sensitivity and throughput of many detection methods.

In our laboratory, we are developing single molecule assays for various biomolecule targets using single-molecule array (Simoa) and digital enzyme-linked immunosorbent assay (digital ELISA). The limit of detection of Simoa technology is typically about a factor of 100 to 1000 times more sensitive than the conventional ELISA, therefore it opens up the potential for being able to measure proteins at concentrations that have never been detected before in various kinds of samples including blood, which is the main focus for Simoa-based assays. (click here to see a recent review about Simoa)

 

Projects: (click to see details)

Development of Ultra-sensitive Single Molecule Array (Simoa) Assays to Directly Detect Mycobacterium Tuberculosis (Mtb) Bacterial Proteins.

Breast Cancer Diagnostics.

Development of Phospho-c-abl and Phospho-CRKL Assays for Use as Nilotinib Companion Diagnostics in Parkinson’s Disease.

Detection of Post-Translationally Modified Proteins as a Biomarker panel for Parkinson’s Disease.

Single Cell Analysis for Cancer Cells.

Ultra-sensitive Detection of MicroRNA.

Developing Simple and Cost-effective Platform for High-throughput Monitoring and Screening Novel Targets in Biological and Biomedical Applications.

Developing Microwell Array Platform to Perform Single Molecule Studies in Fundamental Research Applications: Enzymology, Protein Synthesis etc.