Publications

2020

Plasma IL-6 changes correlate to PD-1 inhibitor responses in NSCLC, Alissa Keegan, Biagio Ricciuti, Padric Garden, Limor Cohen, Reiko Nishihara, Anika Adeni, Cloud Paweletz, Julianna Supplee, Pasi A Jänne, Mariano Severgnini, Mark M. Awad, David R. Walt, Journal for Immunotherapy of Cancer, 2020, doi: 10.1136/jitc-2020-000678

Simplified digital enzyme-linked immunosorbent assay using tyramide signal amplification and fibrin hydrogels, Adam M. Maley, Padric M. Garden, David R. Walt, ACS Sensors, 2020, doi: 10.1021/acssensors.0c01661

Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19, Maia Norman, Tal Gilboa, Alana F. Ogata, Adam M. Maley, Limor Cohen, Evan L. Busch, Roey Lazarovits, Chih-Ping Mao, Yongfei Cai, Jun Zhang, Jared E. Feldman, Blake M. Hauser, Timothy M. Caradonna, Bing Chen, Aaron G. Schmidt, Galit Alter, Richelle C. Charles, Edward T. Ryan, David R. Walt, Nature Biomedical Engineering, 2020, doi: 10.1038/s41551-020-00611-x

Sequential protein capture in multiplex single molecule arrays: a strategy for eliminating assay cross-reactivity, Tal Gilboa, Adam M. Maley, Alana F. Ogata, Connie Wu, David R. Walt, Advanced Healthcare Materials, 2020, doi: 10.1002/adhm.202001111

Ultra-sensitive serial profiling of SARS-CoV-2 antigens and antibodies in plasma to understand disease progression in COVID-19 patients with severe disease, Alana F. Ogata, Adam M. Maley, Connie Wu, Tal Gilboa, Maia Norman, Roey Lazarovits, Chih-Ping Mao, Gail Newton, Matthew Chang, Katrina Nguyen, Maliwan Kamkaew, Quan Zhu, Travis E. Gibson, Edward T. Ryan, Richelle C. Charles, Wayne A. Marasco, David R. Walt, Clinical Chemistry, 2020, doi: 10.1093/clinchem/hvaa213

Ultrasensitive detection of enzymatic activity using single molecule arrays, Xu Wang, Alana F. Ogata, David R. Walt, Journal of the American Chemical Society, 2020, doi: 10.1021/jacs.0c06599

Simultaneous detection of small molecules, proteins, and microRNAs using single molecule arrays, Xu Wang, David R. Walt, Chemical Science, 2020, doi: 10.1039/D0SC02552F

Single-molecule analysis determines isozymes of human alkaline phosphatase in serum, Yu Jiang, Xiang Li, David R. Walt, Angewandte Chemie, 2020, doi: 10.1002/anie.202007477

Single molecule protein detection with attomolar sensitivity using droplet digital enzyme-linked immunosorbent assay, Limor Cohen, Naiwen Cui, Yamen Cai, Padric M. Garden, Xiang Li, David A. Weitz, David R. Walt, ACS Nano, 2020, doi: 10.1021/acsnano.0c02378

Ultrasensitive detection of attomolar protein concentrations by dropcast single molecule assays, Connie Wu, Padric M. Garden, David R. Walt, Journal of the American Chemical Society, 2020, doi: 10.1021/jacs.0c04331

Hypothermic ex situ perfusion of human limbs with acellular solution for 24 hours, Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi, Anamika Veeramani, Obada Abdulrazzak, Bruce Bausk, David R. Walt, Bohdan Pomahac, Transplantation, 2020, doi: 10.1097/tp.0000000000003221

2019

Single-molecule measurements in microwells for clinical applications, Connie Wu, Adam M. Maley, David R. Walt, Critical Reviews in Clinical Laboratory Sciences, 2019, doi: 10.1080/10408363.2019.1700903

Single-molecule mechanistic study of enzyme hysteresis, Yu Jiang, Xiang Li, Barrett R. Morrow, Arti Pothukuchy, Jimmy Gollihar, Richard Novak, Charles B. Reilly, Andrew D. Ellington, David R. Walt, ACS Central Science, 2019, doi: 10.1021/acscentsci.9b00718

A rapid triage test for active pulmonary tuberculosis in adult patients with persistent cough, Rushdy Ahmad, Liangxia Xie, Margaret Pyle, Marta F. Suarez, Tobias Broger, Dan Steinberg, Shaali M. Ame, Marilla G. Lucero, Matthew J. Szucs, Melanie MacMullan, Frode S. Berven, Arup Dutta, Diozele M. Sanvictores, Veronica L. Tallo, Robert Bencher, Dominic P. Eisinger, Usha Dhingra, Saikat Deb, Said M. Ali, Saurabh Mehta, Wafaie W. Fawzi, Ian D. Riley, Sunil Sazawal, Zul Premji, Robert Black, Christopher J. L. Murray, Bill Rodriguez, Steven A. Carr, David R. Walt, Michael A. Gillette, Science Translational Medicine, 2019, doi:10.1126/scitranslmed.aaw8287 

Single-molecule arrays for ultrasensitive detection of blood-based biomarkers for immunotherapy, Limor Cohen, Alissa Keegan, David R. Walt, Methods in Molecular Biology, 2019, doi: 10.1007/978-1-4939-9773-2_18

Clinical testing should be individualized, not based on populations, David R. Walt, The Journal of Clinical Investigation, 2019, doi.org/10.1172/JCI130903

Protein detection by counting molecules, David C. Duffy, David R. Walt, Clinical Chemistry, 2019, DOI:10.1373/clinchem.2018.298653

Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines, Limor Cohen, Alissa Keegan, Stacy E.F. Melanson, David R. Walt, Clinical Biochemistry, 2019, 65, 38-44. doi.org/10.1016/j.clinbiochem.2019.01.001

2018

Competitive Immunoassays for the Detection of Small Molecules Using Single Molecule Arrays, Xu Wang, Limor Cohen, Jun Wang. David R. Walt, Journal of the American Chemical SocietyDOI: 10.1021/jacs.8b11185

Detection of amyloid β oligomers toward early diagnosis of Alzheimer’s disease, Soyoon Sarah Hwang, Hon Chan, Mirco Sorci, James Van Deventer, Dane Wittrup, Georges Belfort, David R. Walt, Analytical Biochemistrydoi.org/10.1016/j.ab.2018.09.011

Finding useful biomarkers for Parkinson’s disease, Science Translational MedicineDOI: 10.1126/scitranslmed.aam6003

Evaluation of Antibody Biotinylation Approaches for Enhanced Sensitivity of Single Molecule Array (Simoa) Immunoassays, Limor Cohen, David R. Walt, Bioconjugate Chemistry. DOI: 10.1021/acs.bioconjchem.8b00601

Highly sensitive and multiplexed protein measurements, Limor Cohen, David R. Walt, Chemical Reviews.  DOI: 10.1021/acs.chemrev.8b00257

Bottom-up single-molecule strategy for understanding subunit function of tetrameric β-galactosidase, Xiang Li, Yu Jiang, Shaorong Chong, David R. Walt, Proceedings of the National Academy of Sciences . 201805690.

How many human proteoforms are there. Nature Chemical Biology. doi:10.1038/nchembio.2576

48 Single Molecule Array Ultra-Sensitive Cytokine Assays in EDTA Plasma Are Robust and Clinically Feasible, Alissa Minkovsky, Limor Cohen, Stacy E. F. Melanson, David R. Walt, American Journal of Clinical Pathology. doi.org/10.1093/ajcp/aqx149.417

Ultra-sensitive single molecule enzyme detection and analysis using a polymer microarray, Barrett K. Duan, Peter E. Cavanagh, Xiang Li, David R. Walt, Analytical Chemistry. DOI: 10.1021/acs.analchem.7b03980

Single Molecule Arrays for ultra-sensitive detection of rat cytokines in serum, Limor Cohen, Liangxia Xie, Mark E. Xylas, David R. Walt, Journal of Immunological Methodsdoi.org/10.1016/j.jim.2017.10.002

2017

Development of a Rapid Salivary Proteomic Platform for Oral Feeding Readiness in the Preterm Newborn, Prarthana Khanna, Jill L. Maron, David R. Walt, Front. Pediatr. doi.org/10.3389/fped.2017.00268

“Using Next-Generation Sequencing to Explore Genetics and Race in the High School Classroom.” Yang, Xinmiao, Mark R. Hartman, Kristin T. Harrington, Candice M. Etson, Matthew B. Fierman, Donna K. Slonim, and David R. Walt.  CBE-Life Sciences Education 16, no. 2 (2017): ar22.

Long-Term Measurements of Human Inflammatory Cytokines Reveal Complex Baseline Variations between Individuals. Wu, D., Dinh, T. L., Bausk, B. P., & Walt, D. R. (2017). The American journal of pathology, 187(12), 2620-2626.

Highly Localized Adaptive Divergence within Supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine, Barney, B.T.; Munkholm, C.; Walt, D.R.; Palumbi, S.R., BMC Genomics, 2017, 18, 271-284. DOI: 10.1186/s12864-017-3660-3.

“Single Molecule Arrays for Protein and Nucleic Acid Analysis”, Cohen, L.; Walt, D.R. Annual Review of Analytical Chemistry, 2017, 10, 345-363.

“Digital direct detection of microRNAs using Single Molecule Arrays” Cohen, L.; Hartman, M.; Amardey-Wellington, A.; Walt, D.R. Nucleic Acids Research, 2017. DOI 10.1093/nar/gkx542 

“Rapid and Ultrasensitive Single Molecule Detection of Botulinum Neurotoxin Serotype A1 in Human Serum and Urine using Single Molecule Array Method”, Dinh, T.L.; Ngan, K.C.; Shoemaker, C.B.; Walt, D.R. Forensic Toxicology, 2017, 35, 179-184. DOI: 10.1007/s11419-016-0336-7

2016

“Using Antigen-Antibody Binding Kinetics Parameters to Understand Single Molecule Array Immunoassay Performance”, Dinh, T.L.; Ngan, K.C.; Shoemaker, C.B.; Walt, D.R., Analytical Chemistry, 2016, 88, 11335-11339. DOI: 10.1021/acs.analchem.6b03192.

“Personal microbiomes and next-generation sequencing for laboratory-based education”, Hartman, M.; Harrington, K.; Etson, C.; Fierman, M.; Slonim, D.; Walt, D., FEMS Microbiology Letters, 2016, 363(23): fnw266. DOI: 10.1093/femsle/fnw266.

“Protein Counting in Single Cancer Cells”, Schubert, S.M.; Walter, S.R.; Manesse, M.; Walt, D.R., Analytical Chemistry, 2016, 88(5), 2952-2957. DOI: 10.1021/acs.analchem.6b00146.

“Incorporation of SOMAmer® Reagents in Single Molecule Array (Simoa) Assays for Cytokine Detection with Ultra-high Sensitivity”, Wu, D.; Katilius, E.; Olivas, E.; Dumont Milutinovic, M.; Walt, D.R., Analytical Chemistry, 2016, 88(17), 8385-8389. DOI: 10.1021/acs.analchem.6b02451.

2015

“Catalytic kinetics of single gold nanoparticles observed via optical microwell arrays,” Mayer, K.M.; Shnipes, J.; Davis, D.; Walt, D.R. Nanotechnology, 2015, 26, 055704. DOI: 10.1088/0957-4484/26/5/055704.

Salivary diagnostics using a portable point-of-service platform: A Review, Khanna, P., Walt, D.R. Clinical Therapeutics, 2015, 37, 498-504. DOI: 10.1016/j.clinthera.2015.02.004. 

“The stoichiometry of the α-complementation reaction of E. coli β-galactosidase as revealed through single-molecule studies”, Mogalisetti, P., Walt, D.R. Biochemistry, 2015, 54, 1583-1588. DOI: 10.1021/bi5015024.

“Single Molecule Arrays (Simoa) for the Ultra-Sensitive Detection of the Host Immune Response to Dengue Infection”, Gaylord, S.; Abdul-Aziz, S.; Walt, D.R., Journal of Clinical Microbiology, 2015, 53, 1722-1724. DOI: 10.1128/JCM.03487-14. PMID: 25694521.

“Fiber-Optic Array Using Molecularly Imprinted Microspheres for Antibiotic Analysis”, Carrasco, S.; Benito-Peña, E.; Walt, D.R.; Moreno-Bondi, M.C., Chemical Science, 2015, 6, 3139-3147. DOI: 10.1039/C5SC00115C.

“Ultra-Sensitive Detection of Ricin Toxin in Multiple Sample Matrices Using Single Domain Antibodies”, Gaylord, S.T.; Goldman, E.R.; Dinh, T.; Anderson, G.P.; Walt, D.R. Analytical Chemistry, 2015, 87, 6570-6577. DOI: 10.1021/acs.analchem.5b00322.

“Ultra-sensitive protein detection via single molecule arrays towards early stage cancer monitoring”, Schubert, S.M.; Arendt, L.M.; Zhou, W.; Baig, S.; Walter, S.R.; Buchsbaum, R.J.; Kuperwasser, C.; Walt, D.R., Scientific Reports, 2015, 5, 11034. DOI: 10.1038/srep11034. PMID: 26052106.

“Single Molecule Array (Simoa) Assay with Optimal Antibody Pairs for Cytokine Detection in Human Serum Samples”, Wu, D.; Dumont Milutinovic, M.; Walt, D.R., Analyst, 2015, 140, 6277-6282. DOI: 10.1039/C5AN01238D.

“Correlations of Salivary Biomarkers with Clinical Assessments in Patients with Cystic Fibrosis”, Nie, S.; Zhang, H.; Mayer, K.M.; Oppenheim, F.G.; Little, F.F.; Greenberg, J.; Uluer, A.Z.; Walt, D.R., PLOS One, 2015, 10(8), e0135237. DOI: 10.1371/journal.pone.0135237. PMCID: PMC4530931.

“Training the Next Generation of Biomedical Investigators in Glycosciences”. Agre, P.; Bertozzi, C.; Bissell, M.J.; Campbell, K.; Cummings, R.; Desai, U.; Estes, M.; Flotte, T.; Fogleman, G.; Gage, F.H.; Ginsburg, D.; Gordon, J.; Hart, G.; Hascall, V.; Kiessling, L.L.; Kornfeld, S.; Lowe, J.B.; Magnani, J.; Mahal, L.; Medzhitov, R.; Roberts, R.J.; Sackstein, R.; Sarkar, R.; Schnaar, R.; Schwartz, N.; Varki, A.; Walt, D.; Weissman, I. National Heart Lung and Blood Institute Working Group Meeting, 15 April 2015, Bethesda, MD. Journal of Clinical Investigation, 2015, 126, 405-408. DOI: 10.1172/JCI85905. PMID: 26829621.

2014

Elucidating the relationship between substrate and inhibitor binding to the active sites of tetrameric β-galactosidase, Mogalisetti, P., Gorris, H. H., Rojek, M. J., & Walt, D. R, Chemical Science5(11), 4467-4473.

“Salivary Inflammatory Mediator Profiling and Correlation to Clinical Disease Markers in Asthma”, Little FF, Delgado DM, Wexler PJ, Oppenheim FG, Mitchell P, Feldman JA, Walt DR, Peng RD, Matsui EC, PLOS One, 2014, 9(1), e84449. DOI: 10.1371/journal.pone.0084449.

“Observing single enzyme molecules interconvert between activity states upon heating”, Rojek, M.J., Walt, D.R., PLOS One, 2014, 9(1), e86224. DOI: 10.1371/journal.pone.0086224.

“Protein Measurements in Microwells”, Walt, David R., Lab on a Chip, 2014, 14, 3195-3200. DOI: 10.1039/C4LC00277F.

“An Automated Integrated Platform for Rapid and Sensitive Multiplexed Protein Profiling Using Human Saliva Samples”, Nie, S.; Henley, W.H.; Miller, S.E.; Zhang, H.; Mayer, K.M.; Dennis, P.J.; Oblath, E.A.; Alarie, J.P.; Wu, Y.; Oppenheim, F.G.; Little, F.F.; Uluer, A.Z.; Wang, P.; Ramsey, J.M.; Walt, D.R. Lab on a Chip, 2014, 14, 1087-98. DOI: 10.1039/c3lc51303c. PMID: 24448498.

“Advancing the Speed, Sensitivity and Accuracy of Biomolecular Detection using Multi-Length Scale Engineering”, Shana Kelley, Chad Mirkin, David Walt, Rustem Ismagilov, Mehmet Toner, and Edward Sargen, Nature Nanotechnology, 2014, 9, 969-980. DOI: 10.1038/nnano.2014.261.

“Genome-Wide SNP-Genotyping Array to Study the Evolution of the Human Pathogen Vibrio vulnificus Biotype 3”, Nili Raz, Yael Danin-Poleg, Ryan B. Hayman, Yudi Bar-On, Alex Linetsky, Michael Shmoish, Eva Sanjua, Carmen Amaro, David R. Walt, Yechezkel Kashi, PLOS One, 2014, 9(12), e114576. DOI: 10.1371/journal.pone.0114576.

“Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers”, Warren, A.D.; Gaylord, S.T.; Ngan, K.C.; Dumont Milutinovic, M.; Kwong, G.A.; Bhatia, S.N.; Walt, D.R. Journal of the American Chemical Society, 2014, 136(39):13709-13714. DOI: 10.1021/ja505676h. PMID: 25198059.

2013

“Optical Methods for Single Molecule Detection and Analysis”, D. R. Walt, Anal. Chem., 2013, 85 (3), pp 1258–1263. DOI: 10.1021/ac3027178.

“Direct detection of bacterial genomic DNA at subfemtomolar concentrations using single molecule arrays” Song, Linan; Shan, Dandan; Zhao, Mingwei; Pink, Brian; Minnehan, Kaitlin; York, Lyndsey; Gardel, Melissa; Sullivan, Sean; Phillips, Aaron; Hayman, Ryan; Walt, David; Duffy, David. Analytical Chemistry, 2013, 85(3):1932-9. DOI: 10.1021/ac303426b.

“Multiplexed fluorescent microarray for human salivary protein analysis using polymer microspheres and fiber-optic bundles”, Nie, S.; Benito-Peña, E.; Zhang, H.; Wu, Y.; Walt, D.R. Journal of Visualized Experiments, 2013 Oct 10(80). DOI: 10.3791/50726. PMID: 24145242.

“Measuring atomic emission from beacons for long-distance chemical signaling”, LaFratta, C.N.; Pelse, I.; Falla, J.L.; Liu, Y.; Palacios, M.A.; Manesse, M.; Whitesides, G.M.; Walt, D.R. Analytical Chemistry, 2013, 85, 8933-8936. DOI: 10.1021/ac402494s. PMID: 23987667.

“Dynamic microbead arrays for biosensing applications”, David R. Walt; Phillips, Aaron; Hayman, Ryan; Lafratta, Christopher; Manesse, Mael; Palacios, Manuel, Lab Chip, 2013, 13 (11), 2153 – 2160. DOI: 10.1039/c3lc00044c. PMID: 23615790.

“Assessing the Stochastic Intermittency of Single Quantum Dot Luminescence for Robust Quantification of Biomolecules”, Palacios, Manuel; Lacy, Michael; Schubert, Stephanie; Manesse, Mael; Walt, David, Analytical Chemistry, 2013, 85 (14), 6639–6645. DOI: 10.1021/ac4001332.

“Multiplexed Salivary Protein Profiling for Patients with Respiratory Diseases using Fiber-Optic Bundles and Fluorescent Antibody-Based Microarrays”, Nie, Shuai; Benito-Peña, Elena; Zhang, Huaibin; Wu, Yue; Walt, David. Analytical Chemistry, 2013, 85 (19), 9272–9280. DOI: 10.1021/ac4019523.

2012

“Single-Molecule Studies of Restriction Endonuclease Kinetics”, Candice Etson, Francis Wilburn, Thomas Moody, Victoria Fashakin, David R. Walt, Biophysical Journal, Volume 102(3), Supplement 1, 1/31/2012.

“Robust Error Correction in Infofuses,” G. Morrison, S.W. Thomas III, C.N. LaFratta, J. Guo, M.A. Palacios, S. Sounkusale, D.R. Walt, G.M. Whitesides, L. Mahadevan. Proceedings of the Royal Society A, 2/8/2012, 468(2138), 361-367 2011

“Transforming Glycoscience: A Roadmap for the Future”. Committee on Assessing the Importance nd Impact of Glycomics and Glycoscinces; Board on Chemical Sciences and Technology; Board on Life Sciences. National Research Council Report. 2012, Washington, D.C.: The National Academies Press.

“Oil-sealed femtoliter fiber-optic arrays for single molecule analysis”, Zhang H., Nie, S., Etson, C.M., Wang, C.M., D. R. Walt, Lab Chip, 2012, 12, 2229-2239. DOI: 10.1039/c2lc21113k. PMID: 22311152.

2011

“Lessons learned from the introduction of personalized genotyping into a medical school curriculum,” D.R. Walt, A. Kuhlik, S. Epstein, L.A. Demmer, M. Knight, D. Chelmow, M. Rosenblatt, D.W. Bianchi. Genetics IN Medicine, 2011, 13, 1, 63-66.

“Artificial Noses,” S. Stitzel, M.J. Aernecke, D.R. Walt. Annual Review of Biomedical Engineering, 2011, 13, 1-25.

“Single-Cell Time-Resolved Quorum Responses Reveal Dependence on Cell Density and Configuration,” R.D. Whitaker, S. Pember, B.C. Wallace, C.E. Brodley, D.R. Walt. The Journal of Biological Chemistry, 2011, 24, 21623-32.

“Whole Saliva Proteolysis and its Important Impact on Salivary Diagnostics,” K. Thomadaki, E.J. Helmerhorst, N. Tian, X. Sun, W.L. Siqueira, D.R. Walt, F.G. Oppenheim. Journal of Dental Research, 2011, 90, 1135-1330. DOI: 10.1177/0022034511420721. PMID: 21917601.

“InfoBiology by Printed Assays of Microorganism Colonies for Timed and On-Demand Release of Messages,” M.A. Palacios, E. Benito-Pena, M.A. Manesse, A. Mazzeo , C.N. LaFratta , G.M. Whitesides, D.R. Walt. Proceeding of the National Academy of Sciences, 2011, 108(40), 16510-4.

2010

“Detection and Classification of Ignitable Liquid Residues using a Fluorescence-Based Vapor-Sensitive Microsphere Array,” M.J. Aernecke, D.R. Walt. Journal of Forensic Sciences 2010, 55(1), 178-84.

“Synthesis and Biological Testing of Penicillins: An Investigative Approach to the Undergraduate Teaching Laboratory,” R.D. Whitaker, L. Truhlar, D. Yuksel, D.R. Walt, M. Williams. Journal of Chemical Education, 2010, 87(6), pp 634-636.

“Analytical Chemistry on the Femtoliter Scale,” H.H. Gorris, D.R. Walt. Angewandte Chemie, 2010, 49, 3880-3895.

“Note: Toward multiple addressable optical trapping,” A. Faustov, M.R. Webb, D.R. Walt. The Review of Scientific Instruments, 2010, 81(2), 026109.

“Fibre optic microarrays,” D.R. Walt. Chem. Soc. Rev., 2010, 39(1), 38-50.

“Visualizing Fluorescence: Using a Homemade Fluorescence “Microscope” to View Latent Fingerprints on Paper,” C.N. LaFratta, S.P. Huh, A. Mallillin, P. Riviello, D.R. Walt. Journal of Chemical Education, 2010, 87(10), 1105-1107.

“Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations,” D.M. Rissin, C.W. Kan, T.G. Campbell, S.C. Stuart, D.R. Fournier, L. Song, T. Piech, P.P. Patel, L. Chang, A.J. Rivnak, E.P. Ferrell, J.D. Randall, G.K. Provuncher, D.R. Walt, D.C. Duffy. Nature Biotechnology, 2010, 28, 595-599.

“Bead-based optical fiber arrays for artificial olfaction,” D.R. Walt. Current Opinion in Chemical Biology, 2010, 14(6), 767-770.

“Toward multiple addressable optical trapping,” A.R. Faustov, M.R. Webb, D.R. Walt. Review of Scientific Instruments, 2010, 81(2, Pt. 1), 026109/1-026109/3.

For publications before 2010, please contact the Walt Lab.